Tris(hydroxymethyl)aminomethane

☑ Tris buffer ha e sebelisoe haholo joalo ka sehlapolli sa li-nucleic acid le liprotheine, empa hape e na le litšebeliso tse ngata tsa bohlokoa.Tris e ne e sebelisetsoa kholo ea kristale ea protheine tlas'a maemo a fapaneng a pH.
☑ Matla a tlase a ionic a Tris buffer a ka sebelisoa ho etsa fiber e mahareng ea lamin ho C. elegans.
☑ Tris hape ke e 'ngoe ea likarolo tsa mantlha tsa protheine electrophoresis buffer.
☑Ho feta moo, Tris ke setsi sa ho lokisa li-surfactants, li-accelerator tsa vulcanization le lithethefatsi tse ling.Tris e boetse e sebelisoa e le tekanyetso ea titration.
☑Trimethylaminomethane e sebelisoa haholo ho acute metabolic le acidemia ea ho hema.Ke alkaline buffer 'me e na le phello e ntle ea buffer ho metabolism acidosis le karabelo ea ts'ebetso ea enzyme.
☑Tris hangata e sebelisoa joalo ka biological buffer, 'me pH ea eona ke 6.8, 7.4, 8.0, 8.8.Sebopeho sa eona sa sebopeho, boleng ba pH bo fetoha haholo ka mocheso.Ka kakaretso, boleng ba pH bo fokotseha ka 0.03 ha mocheso o nyoloha ka tekanyo e le 'ngoe.Tris e sebelisoa haholo ho lokiseng tharollo ea "buffer" litekong tsa biochemistry le molecular biology.Ka mohlala, li-buffers tsa TAE le tbe (tse sebelisoang bakeng sa ho qhibiliha ha nucleic acid) tse sebelisoang hangata litekong tsa biochemical li hloka Tris.Hobane e na le lihlopha tsa amino, e ka arabela ka aldehydes.
☑ Tris ke setsi se fokolang, 'me PKA ea eona ke 8.1 mocheso oa kamore (25 ℃).Ho latela mohopolo oa buffer, mofuta o sebetsang oa buffer oa Tris buffer o pakeng tsa 7.0 le 9.2.The pH boleng ba Tris base aqueous solution ke hoo e ka bang 10.5.Ka kakaretso, hydrochloric acid e eketsoa ho lokisa boleng ba pH ho boleng bo lakatsehang ho fumana tharollo ea buffer ka boleng bona ba pH.Ka nako e ts'oanang, re lokela ho ela hloko phello ea mocheso ho pKa ea Tris.Hobane Tris buffer ke tharollo e fokolang ea alkaline, DNA e tla tlosoa ka har'a tharollo e joalo ho ntlafatsa ho qhibiliha ha eona.Hangata batho ba eketsa EDTA ho Tris hydrochloric acid buffer ho etsa "Te buffer", e sebelisetsoang ho tsitsisa le ho boloka DNA.Haeba tharollo ea asiti ea ho fetola boleng ba pH e nkeloa sebaka ke acetic acid, "Tae buffer" (Tris / acetate / EDTA) ea fumanoa, 'me "tbe buffer" (Tris / borate / EDTA) e fumanoa ka ho e nkela sebaka ka boric acid. .Li-buffer tsena tse peli hangata li sebelisoa litekong tsa nucleic acid electrophoresis.